A research in search of to develop a novel therapeutic technique for periodontitis that focused stem cell senescence by modulating the microenvironmental cues was introduced on the 102nd Basic Session of the IADR, which was held along side the 53rd Annual Assembly of the American Affiliation for Dental, Oral, and Craniofacial Analysis and the forty eighth Annual Assembly of the Canadian Affiliation for Dental Analysis, on March 13-16, 2024, in New Orleans, LA, U.S..
The summary, “Polyphenol-Mediated Oxygenating Hydrogel Ameliorates Periodontitis By Concentrating on Stem Cell Senescence” was introduced throughout the “Biologically Lively Supplies for Restorative and Regenerative Purposes” Poster Session that happened on Friday, March 15, 2024 at 11 a.m. Central Commonplace Time (UTC-6).
The research, by Chengxinyue Ye of Sichuan College, Chengdu, China, constructed a polyphenol-mediated oxygenating hydrogel with polyphenol-mediated manganese modified calcium peroxide (CPO) nanoparticles and polyphenol-modified zeolitic imidazolate framework-8 nanoparticles integrated into silk fibroin methacrylate matrix.
The in vitro biocompatibility, anti-senescent and immunomodulatory impact of the hydrogel was evaluated by making use of on human-derived periodontal ligament stem cells (hPDLSC) in addition to macrophages in a liposaccharide-induced pro-inflammatory microenvironment. The in vivo therapeutic effectiveness was assessed with a rat ligature-induced periodontitis mannequin.
The polyphenol-based modification technique endowed the hydrogel with promoted oxygen manufacturing, antioxidant functionality and reasonable mechanical properties. The hydrogel downregulated markers of senescence (senescence related β-galactosidase exercise, P16 and P21 expression), decreased senescence-associated secretory phenotype expression, scavenged intracellular reactive oxygen species, in addition to promoted proliferation and osteogenic differentiation functionality in infected hPDLSC. The hydrogel additionally exhibited immunomodulatory impact by curbing pro-inflammatory cytokines expression in macrophages and driving the macrophage polarization towards M2 phenotype.
The hydrogel facilitated each alveolar bone and periodontal ligament regeneration in rat periodontitis, presenting a synergistic impact of assuaging native hypoxia, inhibiting inflammatory responses and suppressing mobile senescence. The polyphenol-mediated oxygenating hydrogel successfully boosted periodontal regeneration by reversing the infected and hypoxic microenvironment and suppressing stem cell senescence. This research offered a novel anti-senescent remedy for periodontitis and casted new gentle on potential regenerative materials designing.
Supplied by
Worldwide Affiliation for Dental, Oral, and Craniofacial Analysis
